Journal: bioRxiv

Article Title: A 21□Color Spectral Flow Cytometry Panel for Comprehensive Immunophenotyping of Rat Tissues and a Companion 9□Color T□Cell Functional Assay

doi: 10.64898/2026.10.13.758716

Figure Lengend Snippet: Configuration of ID7000 The SONY ID7000 is equipped with five lasers (355□ nm, 405 □nm, 488 □nm, 561 □nm, and 637□ nm) and 149 detectors, enabling detection of the full fluorochrome emission spectrum.

Article Snippet: All flow cytometry data was acquired or analyzed using ID7000 software (Sony Biotechnology).

Techniques:

Journal: bioRxiv

Article Title: A 21□Color Spectral Flow Cytometry Panel for Comprehensive Immunophenotyping of Rat Tissues and a Companion 9□Color T□Cell Functional Assay

doi: 10.64898/2026.10.13.758716

Figure Lengend Snippet: Instrument configuration for the SONY 5 laser ID7000. Sony ID7000 flow cytometer laser detection channels and fluorescent antibodies. The antibodies and fluorophores used in this panel are indicated on the corresponding detectors.

Article Snippet: All flow cytometry data was acquired or analyzed using ID7000 software (Sony Biotechnology).

Techniques: Flow Cytometry

Journal: bioRxiv

Article Title: A 21□Color Spectral Flow Cytometry Panel for Comprehensive Immunophenotyping of Rat Tissues and a Companion 9□Color T□Cell Functional Assay

doi: 10.64898/2026.10.13.758716

Figure Lengend Snippet: Unmixing and Adjustments: N×N plots viewed with the ID7000™ Unmixing Viewer. Unmixing was performed on the live cell population. Unmixed data with B220-BUV496 depicted as an example on the x-axis and all other fluorochromes plotted on the y-axis. Examples of unmixing errors and corrected unmixing results are indicated by red boxes

Article Snippet: All flow cytometry data was acquired or analyzed using ID7000 software (Sony Biotechnology).

Techniques:

Journal: STAR Protocols

Article Title: Protocol for generating high-quality, 45-color spectral flow cytometry data for unsupervised clustering to investigate aging in human PBMCs

doi: 10.1016/j.xpro.2026.104380

Figure Lengend Snippet: Optimization of a 45-color spectral panel (A) “Spectral Viewer” overview of all 45 fluorescence spectra acquired using a six-laser–configured ID7000 (320, 355, 405, 488, 561, and 637 nm) with 182 detector channels. (B) Spillover spreading matrix calculated in FlowJo V10.10.0 from single stains. (C) Titration of antibodies in human PBMCs. The viability dye Zombie NIR and CD73-biotin/Qdot 525 streptavidin were independently optimized (see for final volumes).

Article Snippet: ID7000 Software V2.1 , Sony , https://www.sonybiotechnology.com/instruments/id7000-spectral-cell-analyzer/software.

Techniques: Fluorescence, Titration

Journal: STAR Protocols

Article Title: Protocol for generating high-quality, 45-color spectral flow cytometry data for unsupervised clustering to investigate aging in human PBMCs

doi: 10.1016/j.xpro.2026.104380

Figure Lengend Snippet: Autofluorescence (AF) unmixing workflow for the 45-color panel (A) Ribbon plot showing the fluorescence spectrum of unstained PBMCs. Autofluorescence is especially excited by the 320 nm and 355 nm lasers. (B) In the ID7000 Software, the “Autofluorescence Finder” tool is employed to identify and unmix the AF of two distinct populations within the AF root gate, excited by the 320 nm and 355 nm lasers. (C) AF-A and AF-B are highlighted in the “Spectral Reference Viewer” among the 45-color + 2 AF spectra. The fluorochromes with the greatest AF overlap are BUV496 (turquoise) and SparkUV 387 (purple). (D) AF unmixing improves population separation by extracting AF contributions to the negative population for CD28-BUV496 and CD14-SparkUV 387.

Article Snippet: ID7000 Software V2.1 , Sony , https://www.sonybiotechnology.com/instruments/id7000-spectral-cell-analyzer/software.

Techniques: Fluorescence, Software